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Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta.

Cingolani G, Bednenko J, Gillespie MT, Gerace L
Mol Cell 10 1345-53 (2002)
Cited: 115 times
EuropePMC logo PMID: 12504010

Abstract

Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importin beta. We solved the crystal structure of a fragment of importin beta-1 (1-485) bound to the nonclassical NLS of PTHrP. The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site. Using a permeabilized cell import assay we demonstrate that importin beta (1-485) can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain, which could have evolved into the modern importin beta superfamily.

Reviews - 1m5n mentioned but not cited (1)

  1. The importin β binding domain as a master regulator of nucleocytoplasmic transport. Lott K, Cingolani G. Biochim Biophys Acta 1813 1578-1592 (2011)

Articles - 1m5n mentioned but not cited (1)



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