› 1mda

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF AN ELECTRON-TRANSFER COMPLEX BETWEEN METHYLAMINE DEHYDROGENASE AND AMICYANIN

Released:
DOI: 10.2210/pdb1mda/pdb
PDB entry 1mda coloured by chain, front view.
PDB entry 1mda coloured by chain, side view.
PDB entry 1mda coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Crystal structure of an electron-transfer complex between methylamine dehydrogenase and amicyanin.
Chen L, Durley R, Poliks BJ, Hamada K, Chen Z, Mathews FS, Davidson VL, Satow Y, Huizinga E, Vellieux FM
Biochemistry 31 4959-64 (1992)
PMID: 1599920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149615 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
METHYLAMINE DEHYDROGENASE (HEAVY SUBUNIT) Chains: H, J
Molecule details ›
Chains: H, J
Length: 368 amino acids
Theoretical weight: 36.87 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
METHYLAMINE DEHYDROGENASE (LIGHT SUBUNIT) Chains: L, M
Molecule details ›
Chains: L, M
Length: 121 amino acids
Theoretical weight: 13.08 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain
Amicyanin Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P22364 (Residues: 29-131; Coverage: 98%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
1 modified residue:
Ligand TRQ 2 x TRQ

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 124.7Å b: 124.7Å c: 247.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 not available not available
Expression system: Not provided

Quick links

Citations

14 review citations

Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions.
Berks et al. (1995)
13 more

18 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
17 more