› 1meg

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

Released:
DOI: 10.2210/pdb1meg/pdb
PDB entry 1meg coloured by chain, front view.
PDB entry 1meg coloured by chain, side view.
PDB entry 1meg coloured by chain, top view.
Source organism: Carica papaya
Primary publication:
Crystal structure of a caricain D158E mutant in complex with E-64.
Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW
FEBS Lett 392 35-9 (1996)
PMID: 8769310

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caricain Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 23.34 KDa
Source organism: Carica papaya
Expression system: Escherichia coli
UniProt:
  • Canonical: P10056 (Residues: 133-348; Coverage: 65%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:
Ligand E64 1 x E64
Ligand EOH 1 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 53.45Å b: 65.33Å c: 64.37Å
α: 90° β: 111.6° γ: 90°
R-values:
R R work R free
0.193 0.193 not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The lysosomal cysteine proteases.
McGrath ME. (1999)
2 more

4 mentions without citation

The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site.
Zhu et al. (2004)
3 more