› 1mi6

Electron Microscopy
12.8Å resolution

Docking of the modified RF2 X-ray structure into the Low Resolution Cryo-EM map of RF2 E.coli 70S Ribosome

Released:
DOI: 10.2210/pdb1mi6/pdb
PDB entry 1mi6 coloured by chain, front view.
PDB entry 1mi6 coloured by chain, side view.
PDB entry 1mi6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A cryo-electron microscopic study of ribosome-bound termination factor RF2.
Rawat UB, Zavialov AV, Sengupta J, Valle M, Grassucci RA, Linde J, Vestergaard B, Ehrenberg M, Frank J
Nature 421 87-90 (2003)
PMID: 12511960
Related structures: EMD-1007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide chain release factor RF2 Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 41.26 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P07012 (Residues: 1-365; Coverage: 100%)
Gene names: JW5847, b2891, prfB, supK
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12.8Å
Relevant EMDB volumes: EMD-1007

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Citations

31 review citations

What recent ribosome structures have revealed about the mechanism of translation.
Schmeing et al. (2009)
30 more

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more