› 1mrc

X-ray diffraction
2.4Å resolution

PREPARATION, CHARACTERIZATION AND CRYSTALLIZATION OF AN ANTIBODY FAB FRAGMENT THAT RECOGNIZES RNA. CRYSTAL STRUCTURES OF NATIVE FAB AND THREE FAB-MONONUCLEOTIDE COMPLEXES

Released:
DOI: 10.2210/pdb1mrc/pdb
PDB entry 1mrc coloured by chain, front view.
PDB entry 1mrc coloured by chain, side view.
PDB entry 1mrc coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Preparation, characterization and crystallization of an antibody Fab fragment that recognizes RNA. Crystal structures of native Fab and three Fab-mononucleotide complexes.
Pokkuluri PR, Bouthillier F, Li Y, Kuderova A, Lee J, Cygler M
J Mol Biol 243 283-97 (1994)
PMID: 7523684

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213051 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IGG2B-KAPPA JEL103 FAB (LIGHT CHAIN) Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.18 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
IGG2B-KAPPA JEL103 FAB (HEAVY CHAIN) Chain: H
Molecule details ›
Chain: H
Length: 215 amino acids
Theoretical weight: 23.02 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 66.4Å b: 75.7Å c: 106.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 not available
Expression system: Not provided

Quick links

Citations

3 review citations

Structure-function correlates of autoantibodies to nucleic acids. Lessons from immunochemical, genetic and structural studies.
Eilat et al. (1994)
2 more

2 mentions without citation

High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors.
Jose et al. (2019)
1 more