1mvw Citations

Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.

J Struct Biol 138 92-104
Related entries: 1m8q, 1o18, 1o19, 1o1a, 1o1b, 1o1c, 1o1d, 1o1e, 1o1f, 1o1g

Cited: 50 times
EuropePMC logo PMID: 12160705

Abstract

Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.

Reviews - 1mvw mentioned but not cited (1)

  1. Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future. Burley SK, Berman HM, Chiu W, Dai W, Flatt JW, Hudson BP, Kaelber JT, Khare SD, Kulczyk AW, Lawson CL, Pintilie GD, Sali A, Vallat B, Westbrook JD, Young JY, Zardecki C. Biophys Rev 14 1281-1301 (2022)

Articles - 1mvw mentioned but not cited (4)



Reviews citing this publication (10)

  1. Structural studies by electron tomography: from cells to molecules. Lucić V, Förster F, Baumeister W. Annu. Rev. Biochem. 74 833-865 (2005)
  2. Electron tomography of membrane-bound cellular organelles. Frey TG, Perkins GA, Ellisman MH. Annu Rev Biophys Biomol Struct 35 199-224 (2006)
  3. Invertebrate muscles: thin and thick filament structure; molecular basis of contraction and its regulation, catch and asynchronous muscle. Hooper SL, Hobbs KH, Thuma JB. Prog. Neurobiol. 86 72-127 (2008)
  4. Large macromolecular complexes in the Protein Data Bank: a status report. Dutta S, Berman HM. Structure 13 381-388 (2005)
  5. Regulation of cytoskeletal dynamics by phospholipase D and phosphatidic acid. Pleskot R, Li J, Zárský V, Potocký M, Staiger CJ. Trends Plant Sci. 18 496-504 (2013)
  6. Mechanical design of translocating motor proteins. Hwang W, Lang MJ. Cell Biochem. Biophys. 54 11-22 (2009)
  7. Multiscale modeling and mechanics of filamentous actin cytoskeleton. Yamaoka H, Matsushita S, Shimada Y, Adachi T. Biomech Model Mechanobiol 11 291-302 (2012)
  8. Protein conformation and molecular order probed by second-harmonic-generation microscopy. Vanzi F, Sacconi L, Cicchi R, Pavone FS. J Biomed Opt 17 060901 (2012)
  9. Evolution of standardization and dissemination of cryo-EM structures and data jointly by the community, PDB, and EMDB. Chiu W, Schmid MF, Pintilie GD, Lawson CL. J Biol Chem 296 100560 (2021)
  10. Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy. Taylor KA, Rahmani H, Edwards RJ, Reedy MK. Int J Mol Sci 20 (2019)

Articles citing this publication (35)



Related citations provided by authors (3)