› 1n2a

X-ray diffraction
1.9Å resolution

Crystal Structure of a Bacterial Glutathione Transferase from Escherichia coli with Glutathione Sulfonate in the Active Site

Released:
DOI: 10.2210/pdb1n2a/pdb
PDB entry 1n2a coloured by chain, front view.
PDB entry 1n2a coloured by chain, side view.
PDB entry 1n2a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conserved structural elements in glutathione transferase homologues encoded in the genome of Escherichia coli.
Rife CL, Parsons JF, Xiao G, Gilliland GL, Armstrong RN
Proteins 53 777-82 (2003)
PMID: 14635120

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase GstA Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9D2 (Residues: 1-201; Coverage: 100%)
Gene names: JW1627, b1635, gst, gstA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTS 2 x GTS
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 47.616Å b: 57.166Å c: 134.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.195 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Glutathione transferases in bacteria.
Allocati et al. (2009)
2 more

3 mentions without citation

Glutathione transferases are structural and functional outliers in the thioredoxin fold.
Atkinson et al. (2009)
2 more