› 1n6m

X-ray diffraction
2.5Å resolution

Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd

Released:
DOI: 10.2210/pdb1n6m/pdb
PDB entry 1n6m coloured by chain, front view.
PDB entry 1n6m coloured by chain, side view.
PDB entry 1n6m coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd.
Yun M, Bronner CE, Park CG, Cha SS, Park HW, Endow SA
EMBO J 22 5382-9 (2003)
PMID: 14532111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-539514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein claret segregational Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 46.47 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20480 (Residues: 293-700; Coverage: 58%)
Gene names: CA(ND), CG7831, ncd
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 162.6Å b: 66.6Å c: 94.8Å
α: 90° β: 98° γ: 90°
R-values:
R R work R free
0.26 0.26 0.302
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Force generation by kinesin and myosin cytoskeletal motor proteins.
Kull et al. (2013)
6 more

19 mentions without citation

Orientation and structure of the Ndc80 complex on the microtubule lattice.
Wilson-Kubalek et al. (2008)
18 more