› 1n8i

X-ray diffraction
2.1Å resolution

Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1n8i/pdb
PDB entry 1n8i coloured by chain, front view.
PDB entry 1n8i coloured by chain, side view.
PDB entry 1n8i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis.
Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Höner zu Bentrup K
J Biol Chem 278 1735-43 (2003)
PMID: 12393860

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase G Chain: A
Molecule details ›
Chain: A
Length: 741 amino acids
Theoretical weight: 80.49 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WK17 (Residues: 1-741; Coverage: 100%)
Gene names: MTCY1A11.06, Rv1837c, glcB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GLV 1 x GLV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 78.095Å b: 78.095Å c: 223.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.185 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Performance of purified antigens for serodiagnosis of pulmonary tuberculosis: a meta-analysis.
Steingart et al. (2009)
10 more

6 mentions without citation

The TB Structural Genomics Consortium: a decade of progress.
Chim et al. (2011)
5 more