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The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.

Vorobiev S, Strokopytov B, Drubin DG, Frieden C, Ono S, Condeelis J, Rubenstein PA, Almo SC
Proc Natl Acad Sci U S A 100 5760-5 (2003)
Related entries: 1d4x, 1nlv, 1nm1, 1yag

Cited: 107 times
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Abstract

The structures of Saccharomyces cerevisiae, Dictyostelium, and Caenorhabditis elegans actin bound to gelsolin segment-1 have been solved and refined at resolutions between 1.9 and 1.75 A. These structures reveal several features relevant to the ATP hydrolytic mechanism, including identification of the nucleophilic water and the roles of Gln-137 and His-161 in positioning and activating the catalytic water, respectively. The involvement of these residues in the catalytic mechanism is consistent with yeast genetics studies. This work highlights both structural and mechanistic similarities with the small and trimeric G proteins and restricts the types of mechanisms responsible for the considerable enhancement of ATP hydrolysis associated with actin polymerization. The conservation of functionalities involved in nucleotide binding and catalysis also provide insights into the mechanistic features of members of the family of actin-related proteins.

Articles - 1nmd mentioned but not cited (5)

  1. The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism. Vorobiev S, Strokopytov B, Drubin DG, Frieden C, Ono S, Condeelis J, Rubenstein PA, Almo SC. Proc Natl Acad Sci U S A 100 5760-5765 (2003)
  2. Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides. Chou SZ, Pollard TD. Proc Natl Acad Sci U S A 116 4265-4274 (2019)
  3. Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP. Nolen BJ, Littlefield RS, Pollard TD. Proc Natl Acad Sci U S A 101 15627-15632 (2004)
  4. Structural basis for the slow dynamics of the actin filament pointed end. Narita A, Oda T, Maéda Y. EMBO J 30 1230-1237 (2011)
  5. Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation. Kumpula EP, Lopez AJ, Tajedin L, Han H, Kursula I. PLoS Biol 17 e3000315 (2019)


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  2. The bacterial cytoskeleton. Shih YL, Rothfield L. Microbiol Mol Biol Rev 70 729-754 (2006)
  3. The evolution of the cytoskeleton. Wickstead B, Gull K. J Cell Biol 194 513-525 (2011)
  4. Invertebrate muscles: muscle specific genes and proteins. Hooper SL, Thuma JB. Physiol Rev 85 1001-1060 (2005)
  5. The ParMRC system: molecular mechanisms of plasmid segregation by actin-like filaments. Salje J, Gayathri P, Löwe J. Nat Rev Microbiol 8 683-692 (2010)
  6. Towards a structural understanding of the remodeling of the actin cytoskeleton. Merino F, Pospich S, Raunser S. Semin Cell Dev Biol 102 51-64 (2020)
  7. Bacterial actins and their diversity. Ozyamak E, Kollman JM, Komeili A. Biochemistry 52 6928-6939 (2013)
  8. Advances in quantum simulations of ATPase catalysis in the myosin motor. Kiani FA, Fischer S. Curr Opin Struct Biol 31 115-123 (2015)
  9. Multiscale simulation of actin filaments and actin-associated proteins. Aydin F, Katkar HH, Voth GA. Biophys Rev 10 1521-1535 (2018)

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