› 1ny6

X-ray diffraction
3.1Å resolution

Crystal structure of sigm54 activator (AAA+ ATPase) in the active state

Released:
DOI: 10.2210/pdb1ny6/pdb
PDB entry 1ny6 coloured by chain, front view.
PDB entry 1ny6 coloured by chain, side view.
PDB entry 1ny6 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains.
Lee SY, De La Torre A, Yan D, Kustu S, Nixon BT, Wemmer DE
Genes Dev 17 2552-63 (2003)
PMID: 14561776

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 267 amino acids
Theoretical weight: 30.62 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 121-387; Coverage: 61%)
Gene names: aq_1117, ntrC1
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 14 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 106.788Å b: 108.265Å c: 110.02Å
α: 70.25° β: 85.9° γ: 73.27°
R-values:
R R work R free
0.272 0.266 0.329
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
25 more

18 mentions without citation

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
17 more