› 1od1

X-ray diffraction
1.37Å resolution

Endothiapepsin PD135,040 complex

Released:
DOI: 10.2210/pdb1od1/pdb
PDB entry 1od1 coloured by chain, front view.
PDB entry 1od1 coloured by chain, side view.
PDB entry 1od1 coloured by chain, top view.
Source organism: Cryphonectria parasitica
Primary publication:
The structure of endothiapepsin complexed with the gem-diol inhibitor PD-135,040 at 1.37 A.
Coates L, Erskine PT, Mall S, Williams PA, Gill RS, Wood SP, Cooper JB
Acta Crystallogr D Biol Crystallogr 59 978-81 (2003)
PMID: 12777758

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with specificity similar to that of pepsin A, prefers hydrophobic residues at P1 and P1', but does not cleave 14-Ala-|-Leu-15 in the B chain of insulin or Z-Glu-Tyr. Clots milk.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endothiapepsin Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 33.81 KDa
Source organism: Cryphonectria parasitica
UniProt:
  • Canonical: P11838 (Residues: 90-419; Coverage: 83%)
Gene names: EAPA, EPN-1
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

2 bound ligands:
Ligand 0QS 1 x 0QS
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 53.201Å b: 73.249Å c: 45.993Å
α: 90° β: 110.1° γ: 90°
R-values:
R R work R free
0.125 not available 0.159

Quick links

Citations

2 citation in other articles

New organofluorine building blocks: inhibition of the malarial aspartic proteases plasmepsin II and IV by alicyclic alpha,alpha-difluoroketone hydrates.
Fäh et al. (2009)
1 more

2 mentions without citation

Trapping a 96 degrees domain rotation in two distinct conformations by engineered disulfide bridges.
Schultz-Heienbrok et al. (2004)
1 more