› 1oeo

X-ray diffraction
2.15Å resolution

PTP1B with the catalytic cysteine oxidized to sulfonic acid

Released:
DOI: 10.2210/pdb1oeo/pdb
PDB entry 1oeo coloured by chain, front view.
PDB entry 1oeo coloured by chain, side view.
PDB entry 1oeo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Redox regulation of protein tyrosine phosphatase 1B involves a sulphenyl-amide intermediate.
Salmeen A, Andersen JN, Myers MP, Meng TC, Hinks JA, Tonks NK, Barford D
Nature 423 769-73 (2003)
PMID: 12802338

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: X
Molecule details ›
Chain: X
Length: 321 amino acids
Theoretical weight: 37.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 88.365Å b: 88.365Å c: 104.764Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.217
Expression system: Escherichia coli

Quick links

Citations

259 review citations

Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling.
Ray et al. (2012)
258 more

4 mentions without citation

Covalent inhibition of protein tyrosine phosphatases.
Ruddraraju et al. (2017)
3 more