1or4 Citations

Structure of the oxygen sensor in Bacillus subtilis: signal transduction of chemotaxis by control of symmetry.

Structure 11 1097-110 (2003)
Cited: 64 times
EuropePMC logo PMID: 12962628

Abstract

Much is now known about chemotaxis signaling transduction for Escherichia coli and Salmonella typhimurium. The mechanism of chemotaxis of Bacillus subtilis is, in a sense, reversed. Attractant binding strengthens the activity of histidine kinase in B. subtilis, instead of an inhibition reaction. The HemAT from B. subtilis can detect oxygen and transmit the signal to regulatory proteins that control the direction of flagella rotation. We have determined the crystal structures of the HemAT sensor domain in liganded and unliganded forms at 2.15 A and 2.7 A resolution, respectively. The liganded structure reveals a highly symmetrical organization. Tyrosine70 shows distinct conformational changes on one subunit when ligands are removed. Our study suggests that disruption of the symmetry of HemAT plays an important role in initiating the chemotaxis signaling transduction cascade.

Reviews - 1or4 mentioned but not cited (3)

  1. Sensory Repertoire of Bacterial Chemoreceptors. Ortega Á, Zhulin IB, Krell T. Microbiol Mol Biol Rev 81 e00033-17 (2017)
  2. Heme-based globin-coupled oxygen sensors: linking oxygen binding to functional regulation of diguanylate cyclase, histidine kinase, and methyl-accepting chemotaxis. Martínková M, Kitanishi K, Shimizu T. J Biol Chem 288 27702-27711 (2013)
  3. Mechanism and Role of Globin-Coupled Sensor Signalling. Walker JA, Rivera S, Weinert EE. Adv Microb Physiol 71 133-169 (2017)

Articles - 1or4 mentioned but not cited (10)



Reviews citing this publication (10)

  1. Diversity in chemotaxis mechanisms among the bacteria and archaea. Szurmant H, Ordal GW. Microbiol Mol Biol Rev 68 301-319 (2004)
  2. Bacterial redox sensors. Green J, Paget MS. Nat Rev Microbiol 2 954-966 (2004)
  3. Sensor complexes regulating two-component signal transduction. Szurmant H, White RA, Hoch JA. Curr Opin Struct Biol 17 706-715 (2007)
  4. Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis. Wuichet K, Alexander RP, Zhulin IB. Methods Enzymol 422 1-31 (2007)
  5. Bacterial sensors of oxygen. Green J, Crack JC, Thomson AJ, LeBrun NE. Curr Opin Microbiol 12 145-151 (2009)
  6. Methyl-accepting chemotaxis proteins: a core sensing element in prokaryotes and archaea. Salah Ud-Din AIM, Roujeinikova A. Cell Mol Life Sci 74 3293-3303 (2017)
  7. Neuroglobin: From structure to function in health and disease. Ascenzi P, di Masi A, Leboffe L, Fiocchetti M, Nuzzo MT, Brunori M, Marino M. Mol Aspects Med 52 1-48 (2016)
  8. Internal sense of direction: sensing and signaling from cytoplasmic chemoreceptors. Collins KD, Lacal J, Ottemann KM. Microbiol Mol Biol Rev 78 672-684 (2014)
  9. Metal-containing sensor proteins sensing diatomic gas molecules. Aono S. Dalton Trans 3137-3146 (2008)
  10. Structural heterogeneity and ligand gating in ferric Methanosarcina acetivorans protoglobin mutants. Pesce A, Tilleman L, Dewilde S, Ascenzi P, Coletta M, Ciaccio C, Bruno S, Moens L, Bolognesi M, Nardini M. IUBMB Life 63 287-294 (2011)

Articles citing this publication (41)