1owx Citations

Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element.

Structure 11 833-43 (2003)
Cited: 77 times
EuropePMC logo PMID: 12842046

Abstract

The La protein is an important component of ribonucleoprotein complexes that acts mainly as an RNA chaperone to facilitate correct processing and maturation of RNA polymerase III transcripts, but can also stimulate translation initiation. We report here the structure of the C-terminal domain of human La, which comprises an atypical RNA recognition motif (La225-334) and a long unstructured C-terminal tail. The central beta sheet of La225-334 reveals novel features: the putative RNA binding surface is formed by a five-stranded beta sheet and, strikingly, is largely obscured by a long C-terminal alpha helix that encompasses a recently identified nuclear retention element. Contrary to previous observations, we find that the La protein does not contain a dimerization domain.

Reviews - 1owx mentioned but not cited (3)

  1. The La and related RNA-binding proteins (LARPs): structures, functions, and evolving perspectives. Maraia RJ, Mattijssen S, Cruz-Gallardo I, Conte MR. Wiley Interdiscip Rev RNA 8 (2017)
  2. xRRM: a new class of RRM found in the telomerase La family protein p65. Singh M, Choi CP, Feigon J. RNA Biol 10 353-359 (2013)
  3. The La-related proteins: structures and interactions of a versatile superfamily of RNA-binding proteins. Dock-Bregeon AC, Lewis KA, Conte MR. RNA Biol 18 178-193 (2021)

Articles - 1owx mentioned but not cited (16)



Reviews citing this publication (14)

  1. RNA-binding proteins: modular design for efficient function. Lunde BM, Moore C, Varani G. Nat Rev Mol Cell Biol 8 479-490 (2007)
  2. The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression. Maris C, Dominguez C, Allain FH. FEBS J 272 2118-2131 (2005)
  3. The role of structural disorder in the function of RNA and protein chaperones. Tompa P, Csermely P. FASEB J 18 1169-1175 (2004)
  4. Conserved and divergent features of the structure and function of La and La-related proteins (LARPs). Bayfield MA, Yang R, Maraia RJ. Biochim Biophys Acta 1799 365-378 (2010)
  5. Sjögren's syndrome--study of autoantigens and autoantibodies. Routsias JG, Tzioufas AG. Clin Rev Allergy Immunol 32 238-251 (2007)
  6. Drug discovery for a new generation of covalent drugs. Kalgutkar AS, Dalvie DK. Expert Opin Drug Discov 7 561-581 (2012)
  7. Autoimmune response and target autoantigens in Sjogren's syndrome. Routsias JG, Tzioufas AG. Eur J Clin Invest 40 1026-1036 (2010)
  8. Role of the RNA-binding protein La in cancer pathobiology. Sommer G, Heise T. RNA Biol 18 218-236 (2021)
  9. A terminal affair: 3'-end recognition by the human La protein. Curry S, Conte MR. Trends Biochem Sci 31 303-305 (2006)
  10. LARP1 and LARP4: up close with PABP for mRNA 3' poly(A) protection and stabilization. Mattijssen S, Kozlov G, Fonseca BD, Gehring K, Maraia RJ. RNA Biol 18 259-274 (2021)
  11. Stabilize and connect: the role of LARP7 in nuclear non-coding RNA metabolism. Hasler D, Meister G, Fischer U. RNA Biol 18 290-303 (2021)
  12. La proteins couple use of sequence-specific and non-specific binding modes to engage RNA substrates. Bayfield MA, Vinayak J, Kerkhofs K, Mansouri-Noori F. RNA Biol 18 168-177 (2021)
  13. NMR in pharmacokinetic and pharmacodynamic profiling. Sun C, Huth JR, Hajduk PJ. Chembiochem 6 1592-1600 (2005)
  14. RBM22, a Key Player of Pre-mRNA Splicing and Gene Expression Regulation, Is Altered in Cancer. Soubise B, Jiang Y, Douet-Guilbert N, Troadec MB. Cancers (Basel) 14 643 (2022)

Articles citing this publication (44)