› 1p7w

X-ray diffraction
1.02Å resolution

Crystal structure of the complex of Proteinase K with a designed heptapeptide inhibitor Pro-Ala-Pro-Phe-Ala-Ser-Ala at atomic resolution

Released:
DOI: 10.2210/pdb1p7w/pdb
PDB entry 1p7w coloured by chain, front view.
PDB entry 1p7w coloured by chain, side view.
PDB entry 1p7w coloured by chain, top view.
Source organism: Parengyodontium album
Entry authors: Bilgrami S, Perbandt M, Chandra V, Banumathi S, Kaur P, Betzel C, Singh TP

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139278 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.96 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
inhibitor peptide Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 660 Da
Source organism: Parengyodontium album
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand NO3 5 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 67.835Å b: 67.835Å c: 101.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.129 0.142
Expression system: Not provided

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