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Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F.

Mukhopadhyay D, Sen U, Zapf J, Varughese KI
Acta Crystallogr D Biol Crystallogr 60 638-45 (2004)
Cited: 12 times
EuropePMC logo PMID: 15039551

Abstract

As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex.

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Articles citing this publication (6)

  1. A combination of glycerol and manganese promotes biofilm formation in Bacillus subtilis via histidine kinase KinD signaling. Shemesh M, Chai Y. J Bacteriol 195 2747-2754 (2013)
  2. High-resolution protein complexes from integrating genomic information with molecular simulation. Schug A, Weigt M, Onuchic JN, Hwa T, Szurmant H. Proc Natl Acad Sci U S A 106 22124-22129 (2009)
  3. Conformational changes of Spo0F along the phosphotransfer pathway. Varughese KI. J Bacteriol 187 8221-8227 (2005)
  4. Structural analysis of divalent metals binding to the Bacillus subtilis response regulator Spo0F: the possibility for in vitro metalloregulation in the initiation of sporulation. Kojetin DJ, Thompson RJ, Benson LM, Naylor S, Waterman J, Davies KG, Opperman CH, Stephenson K, Hoch JA, Cavanagh J. Biometals 18 449-466 (2005)
  5. Metal-dependent SpoIIE oligomerization stabilizes FtsZ during asymmetric division in Bacillus subtilis. Cendrowicz E, de Sousa Borges A, Kopacz M, Scheffers DJ. PLoS One 12 e0174713 (2017)
  6. Enhanced Degradation of Naproxen by Immobilization of Bacillus thuringiensis B1(2015b) on Loofah Sponge. Dzionek A, Wojcieszyńska D, Adamczyk-Habrajska M, Guzik U. Molecules 25 E872 (2020)


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