› 1pl4

X-ray diffraction
1.47Å resolution

Crystal Structure of human MnSOD Y166F mutant

Released:
DOI: 10.2210/pdb1pl4/pdb
Model geometry
Fit model/data
PDB entry 1pl4 coloured by chain, front view.
PDB entry 1pl4 coloured by chain, side view.
PDB entry 1pl4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Amino acid substitution at the dimeric interface of human manganese superoxide dismutase.
Hearn AS, Fan L, Lepock JR, Luba JP, Greenleaf WB, Cabelli DE, Tainer JA, Nick HS, Silverman DN
J Biol Chem 279 5861-6 (2004)
PMID: 14638684

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 198 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04179 (Residues: 25-222; Coverage: 89%)
Gene name: SOD2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 73.689Å b: 77.711Å c: 135.795Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.185 0.237
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
2 more

6 mentions without citation

The structure-function relationships and physiological roles of MnSOD mutants.
Bonetta Valentino R. (2022)
5 more