› 1pnl

X-ray diffraction
2.5Å resolution

PENICILLIN ACYLASE HAS A SINGLE-AMINO-ACID CATALYTIC CENTRE

Released:
DOI: 10.2210/pdb1pnl/pdb
PDB entry 1pnl coloured by chain, front view.
PDB entry 1pnl coloured by chain, side view.
PDB entry 1pnl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Penicillin acylase has a single-amino-acid catalytic centre.
Duggleby HJ, Tolley SP, Hill CP, Dodson EJ, Dodson G, Moody PC
Nature 373 264-8 (1995)
PMID: 7816145

Function and Biology Details

Reaction catalysed: 
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-529871 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Penicillin G acylase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.84 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 27-235; Coverage: 26%)
Gene name: pac
Structure domains:
Penicillin G acylase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 557 amino acids
Theoretical weight: 62.43 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 290-846; Coverage: 68%)
Gene name: pac
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand PAC 1 x PAC
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 52.12Å b: 65.08Å c: 76.3Å
α: 100.2° β: 111.44° γ: 105.81°
R-values:
R R work R free
0.152 not available 0.215

Quick links

Citations

37 review citations

The 26S proteasome: a molecular machine designed for controlled proteolysis.
Voges et al. (1999)
36 more

4 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
3 more