› 1pu8

X-ray diffraction
2.13Å resolution

Crystal structure of H.pylori 3-methyladenine DNA glycosylase (MagIII) bound to 1,N6-ethenoadenine

Released:
DOI: 10.2210/pdb1pu8/pdb
PDB entry 1pu8 coloured by chain, front view.
PDB entry 1pu8 coloured by chain, side view.
PDB entry 1pu8 coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases.
Eichman BF, O'Rourke EJ, Radicella JP, Ellenberger T
EMBO J 22 4898-909 (2003)
PMID: 14517230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HhH-GPD domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.22 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: O25323 (Residues: 1-218; Coverage: 100%)
Gene name: HP_0602
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EA1 2 x EA1
Ligand BME 2 x BME
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: C2
Unit cell:
a: 146.605Å b: 44.399Å c: 81.516Å
α: 90° β: 106.4° γ: 90°
R-values:
R R work R free
0.204 0.202 0.23
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Base excision repair.
Krokan et al. (2013)
9 more

2 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
1 more