› 1q9j

X-ray diffraction
2.75Å resolution

Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1q9j/pdb
PDB entry 1q9j coloured by chain, front view.
PDB entry 1q9j coloured by chain, side view.
PDB entry 1q9j coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis.
Buglino J, Onwueme KC, Ferreras JA, Quadri LE, Lima CD
J Biol Chem 279 30634-42 (2004)
PMID: 15123643

Function and Biology Details

Reaction catalysed: 
2 a mycocerosyl-[mycocerosic acid synthase] + a phenolphthiocerol = a dimycocerosyl phenolphthiocerol + 2 holo-[mycocerosic acid synthase]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phthiocerol/phthiodiolone dimycocerosyl transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 45.47 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WIN5 (Residues: 1-422; Coverage: 100%)
Gene names: MTCY19H9.07, Rv2939, papA5
Sequence domains: Phthiocerol/phthiodiolone dimycocerosyl transferase C-terminus
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A, NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 172.982Å b: 172.982Å c: 80.535Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
7 more

6 mentions without citation

High-throughput computational and experimental techniques in structural genomics.
Chance et al. (2004)
5 more