› 1qdn

X-ray diffraction
2.3Å resolution

AMINO TERMINAL DOMAIN OF THE N-ETHYLMALEIMIDE SENSITIVE FUSION PROTEIN (NSF)

Released:
DOI: 10.2210/pdb1qdn/pdb
PDB entry 1qdn coloured by chain, front view.
PDB entry 1qdn coloured by chain, side view.
PDB entry 1qdn coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein.
May AP, Misura KM, Whiteheart SW, Weis WI
Nat Cell Biol 1 175-82 (1999)
PMID: 10559905

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-148436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vesicle-fusing ATPase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 204 amino acids
Theoretical weight: 22.51 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 1-203; Coverage: 27%)
Gene name: NSF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand BME 6 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P41212
Unit cell:
a: 101.9Å b: 101.9Å c: 126.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.26
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The mechanisms of vesicle budding and fusion.
Bonifacino et al. (2004)
18 more

7 mentions without citation

Cellular functions of NSF: not just SNAPs and SNAREs.
Zhao et al. (2007)
6 more