› 1qfw

X-ray diffraction
3.5Å resolution

TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT

Released:
DOI: 10.2210/pdb1qfw/pdb
Model geometry
Fit model/data
Data not analysed
PDB entry 1qfw coloured by chain, front view.
PDB entry 1qfw coloured by chain, side view.
PDB entry 1qfw coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a ternary complex between human chorionic gonadotropin (hCG) and two Fv fragments specific for the alpha and beta-subunits.
Tegoni M, Spinelli S, Verhoeyen M, Davis P, Cambillau C
J Mol Biol 289 1375-85 (1999)
PMID: 10373373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134115 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Glycoprotein hormones alpha chain Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 25-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Structure domains: Cystine-knot cytokines
Choriogonadotropin subunit beta 3 Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DN86 (Residues: 21-165; Coverage: 100%)
Gene names: CGB, CGB3, CGB5, CGB8
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
Ig kappa chain V-III region PC 3741/TEPC 111 Chain: L
Ig heavy chain V region 102 Chain: H
Ig kappa chain V-III region PC 3741/TEPC 111 Chain: M
Ig heavy chain V region 345 Chain: I

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 104.9Å b: 104.9Å c: 150.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.26 0.31
Expression system: Not provided

Quick links

Citations

12 review citations

Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships.
Szkudlinski et al. (2002)
11 more

22 mentions without citation

Structural-Functional Features of the Thyrotropin Receptor: A Class A G-Protein-Coupled Receptor at Work.
Kleinau et al. (2017)
21 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO