› 1qk4

X-ray diffraction
1.9Å resolution

TOXOPLASMA GONDII HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IMP COMPLEX

Released:
DOI: 10.2210/pdb1qk4/pdb
PDB entry 1qk4 coloured by chain, front view.
PDB entry 1qk4 coloured by chain, side view.
PDB entry 1qk4 coloured by chain, top view.
Source organism: Toxoplasma gondii RH
Primary publication:
Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex.
Héroux A, White EL, Ross LJ, Borhani DW
Biochemistry 38 14485-94 (1999)
PMID: 10545170

Function and Biology Details

Reactions catalysed: 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine-xanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 26.71 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26997 (Residues: 1-230; Coverage: 100%)
Gene name: HPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand IMP 4 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 84.537Å b: 102.445Å c: 108.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 citation in other articles

Substrate deformation in a hypoxanthine-guanine phosphoribosyltransferase ternary complex: the structural basis for catalysis.
Héroux et al. (2000)
26 more

2 mentions without citation

AlphaSpace 2.0: Representing Concave Biomolecular Surfaces Using β-Clusters.
Katigbak et al. (2020)
1 more