› 1qku

X-ray diffraction
3.2Å resolution

WILD TYPE ESTROGEN NUCLEAR RECEPTOR LIGAND BINDING DOMAIN COMPLEXED WITH ESTRADIOL

Released:
DOI: 10.2210/pdb1qku/pdb
PDB entry 1qku coloured by chain, front view.
PDB entry 1qku coloured by chain, side view.
PDB entry 1qku coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a mutant hERalpha ligand-binding domain reveals key structural features for the mechanism of partial agonism.
Gangloff M, Ruff M, Eiler S, Duclaud S, Wurtz JM, Moras D
J Biol Chem 276 15059-65 (2001)
PMID: 11278577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Estrogen receptor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 250 amino acids
Theoretical weight: 28.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03372 (Residues: 301-550; Coverage: 42%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand EST 3 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P3221
Unit cell:
a: 105.5Å b: 105.5Å c: 136.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.275
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Estrogen receptors: how do they signal and what are their targets.
Heldring et al. (2007)
10 more

26 mentions without citation

Structural underpinnings of oestrogen receptor mutations in endocrine therapy resistance.
Katzenellenbogen et al. (2018)
25 more