› 1qlg

X-ray diffraction
2.2Å resolution

Crystal structure of phytase with magnesium from Bacillus amyloliquefaciens

Released:
DOI: 10.2210/pdb1qlg/pdb
Model geometry
Fit model/data
PDB entry 1qlg coloured by chain, front view.
PDB entry 1qlg coloured by chain, side view.
PDB entry 1qlg coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Ha NC, Oh BC, Shin S, Kim HJ, Oh TK, Kim YO, Choi KY, Oh BH
Nat Struct Biol 7 147-53 (2000)
PMID: 10655618

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phytase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 39.01 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: O66037 (Residues: 29-383; Coverage: 99%)
Gene name: phy
Sequence domains: Phytase
Structure domains: TolB, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 64.04Å c: 105.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.265
Expression system: Bacillus subtilis

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Citations

9 review citations

The term phytase comprises several different classes of enzymes.
Mullaney et al. (2003)
8 more

6 mentions without citation

Research status of Bacillus phytase.
Zhao et al. (2021)
5 more