› 1qpc

X-ray diffraction
1.6Å resolution

STRUCTURAL ANALYSIS OF THE LYMPHOCYTE-SPECIFIC KINASE LCK IN COMPLEX WITH NON-SELECTIVE AND SRC FAMILY SELECTIVE KINASE INHIBITORS

Released:
DOI: 10.2210/pdb1qpc/pdb
PDB entry 1qpc coloured by chain, front view.
PDB entry 1qpc coloured by chain, side view.
PDB entry 1qpc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the lymphocyte-specific kinase Lck in complex with non-selective and Src family selective kinase inhibitors.
Zhu X, Kim JL, Newcomb JR, Rose PE, Stover DR, Toledo LM, Zhao H, Morgenstern KA
Structure 7 651-61 (1999)
PMID: 10404594

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase Lck Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 32.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P06239 (Residues: 231-509; Coverage: 55%)
Gene name: LCK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ANP 1 x ANP
Ligand EDO 1 x EDO
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 42.11Å b: 73.71Å c: 91.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.23
Expression system: Trichoplusia ni

Quick links

Citations

23 review citations

Protein tyrosine kinase structure and function.
Hubbard et al. (2000)
22 more

15 mentions without citation

Small molecule substrate phosphorylation site inhibitors of protein kinases: approaches and challenges.
Breen et al. (2015)
14 more