› 1qxo

X-ray diffraction
2Å resolution

Crystal structure of Chorismate synthase complexed with oxidized FMN and EPSP

Released:
DOI: 10.2210/pdb1qxo/pdb
PDB entry 1qxo coloured by chain, front view.
PDB entry 1qxo coloured by chain, side view.
PDB entry 1qxo coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
The structure of chorismate synthase reveals a novel flavin binding site fundamental to a unique chemical reaction.
Maclean J, Ali S
Structure 11 1499-511 (2003)
PMID: 14656434

Function and Biology Details

Reaction catalysed: 
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 43.49 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2Y6 (Residues: 1-388; Coverage: 100%)
Gene names: SP_1374, aroC
Sequence domains: Chorismate synthase
Structure domains: Chorismate synthase AroC

Ligands and Environments


Cofactor: Ligand FMN 6 x FMN
3 bound ligands:
Ligand NCO 9 x NCO
Ligand EDO 7 x EDO
Ligand EPS 4 x EPS
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 81.059Å b: 124.582Å c: 85.163Å
α: 90° β: 115.15° γ: 90°
R-values:
R R work R free
0.16 0.157 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
2 more

6 mentions without citation

Noncanonical reactions of flavoenzymes.
Sobrado P. (2012)
5 more