› 1r5u

X-ray diffraction
4.5Å resolution

RNA POLYMERASE II TFIIB COMPLEX

Released:
DOI: 10.2210/pdb1r5u/pdb
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Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.
Bushnell DA, Westover KD, Davis RE, Kornberg RD
Science 303 983-8 (2004)
PMID: 14963322

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137357 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
DNA-directed RNA polymerase II subunit RPB1 Chain: A
Molecule details ›
Chain: A
Length: 1733 amino acids
Theoretical weight: 191.82 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04050 (Residues: 1-1733; Coverage: 100%)
Gene names: D2150, RPB1, RPB220, RPO21, SUA8, YDL140C
Sequence domains:
DNA-directed RNA polymerase II subunit RPB2 Chain: B
Molecule details ›
Chain: B
Length: 1224 amino acids
Theoretical weight: 138.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08518 (Residues: 1-1224; Coverage: 100%)
Gene names: RPB150, RPB2, RPO22, YOR151C
Sequence domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
TRANSCRIPTION FACTOR II B (TFIIB) Chain: M

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 212.438Å b: 217.182Å c: 422.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.345 0.345 0.373
Expression system: Escherichia coli

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Citations

47 review citations

Nucleosome positioning and gene regulation: advances through genomics.
Jiang et al. (2009)
46 more

12 mentions without citation

Structural perspective on mutations affecting the function of multisubunit RNA polymerases.
Trinh et al. (2006)
11 more

PDB-REDO

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