› 1rg5

X-ray diffraction
2.5Å resolution

Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides carotenoidless strain R-26.1

Released:
DOI: 10.2210/pdb1rg5/pdb
PDB entry 1rg5 coloured by chain, front view.
PDB entry 1rg5 coloured by chain, side view.
PDB entry 1rg5 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Protein regulation of carotenoid binding; gatekeeper and locking amino acid residues in reaction centers of Rhodobacter sphaeroides.
Roszak AW, McKendrick K, Gardiner AT, Mitchell IA, Isaacs NW, Cogdell RJ, Hashimoto H, Frank HA
Structure 12 765-73 (2004)
PMID: 15130469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 2 x U10
Ligand HTO 2 x HTO
Ligand FE 1 x FE
Ligand CDL 1 x CDL
Ligand LDA 6 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P3121
Unit cell:
a: 144.033Å b: 144.033Å c: 190.072Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.155 0.182

Quick links

Citations

5 review citations

Biogenesis, molecular regulation and function of plant isoprenoids.
Bouvier et al. (2005)
4 more

2 mentions without citation

Ensemble refinement of protein crystal structures: validation and application.
Levin et al. (2007)
1 more