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Structure of protein phosphatase methyltransferase 1 (PPM1), a leucine carboxyl methyltransferase involved in the regulation of protein phosphatase 2A activity.

Leulliot N, Quevillon-Cheruel S, Sorel I, Li de La Sierra-Gallay I, Collinet B, Graille M, Blondeau K, Bettache N, Poupon A, Janin J, van Tilbeurgh H
J Biol Chem 279 8351-8 (2004)
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Cited: 57 times
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Abstract

The important role of the serine/threonine protein phosphatase 2A (PP2A) in various cellular processes requires a precise and dynamic regulation of PP2A activity, localization, and substrate specificity. The regulation of the function of PP2A involves the reversible methylation of the COOH group of the C-terminal leucine of the catalytic subunit, which, in turn, controls the enzyme's heteromultimeric composition and confers different protein recognition and substrate specificity. We have determined the structure of PPM1, the yeast methyltransferase responsible for methylation of PP2A. The structure of PPM1 reveals a common S-adenosyl-l-methionine-dependent methyltransferase fold, with several insertions conferring the specific function and substrate recognition. The complexes with the S-adenosyl-l-methionine methyl donor and the S-adenosyl-l-homocysteine product and inhibitor unambiguously revealed the co-substrate binding site and provided a convincing hypothesis for the PP2A C-terminal peptide binding site. The structure of PPM1 in a second crystal form provides clues to the dynamic nature of the PPM1/PP2A interaction.

Articles - 1rje mentioned but not cited (1)

  1. Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4. Suzuki Y, Noma A, Suzuki T, Ishitani R, Nureki O. Nucleic Acids Res 37 2910-2925 (2009)


Reviews citing this publication (24)

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Articles citing this publication (32)

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  6. Coral life history and symbiosis: functional genomic resources for two reef building Caribbean corals, Acropora palmata and Montastraea faveolata. Schwarz JA, Brokstein PB, Voolstra C, Terry AY, Manohar CF, Miller DJ, Szmant AM, Coffroth MA, Medina M. BMC Genomics 9 97 (2008)
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  11. Retracted: Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Morita K, He S, Nowak RP, Wang J, Zimmerman MW, Fu C, Durbin AD, Martel MW, Prutsch N, Gray NS, Fischer ES, Look AT. Cell 181 702-715.e20 (2020)
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  13. Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells. Sontag JM, Nunbhakdi-Craig V, Mitterhuber M, Ogris E, Sontag E. J Neurochem 115 1455-1465 (2010)
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  15. Antiphospholipid antibodies induce thrombosis by PP2A activation via apoER2-Dab2-SHC1 complex formation in endothelium. Sacharidou A, Chambliss KL, Ulrich V, Salmon JE, Shen YM, Herz J, Hui DY, Terada LS, Shaul PW, Mineo C. Blood 131 2097-2110 (2018)
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  17. Vitamin D, Homocysteine, and Folate in Subcortical Vascular Dementia and Alzheimer Dementia. Moretti R, Caruso P, Dal Ben M, Conti C, Gazzin S, Tiribelli C. Front Aging Neurosci 9 169 (2017)
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  21. S-Adenosylmethionine and methylthioadenosine inhibit β-catenin signaling by multiple mechanisms in liver and colon cancer. Li TW, Peng H, Yang H, Kurniawidjaja S, Panthaki P, Zheng Y, Mato JM, Lu SC. Mol Pharmacol 87 77-86 (2015)
  22. Cornel Iridoid Glycoside Inhibits Tau Hyperphosphorylation via Regulating Cross-Talk Between GSK-3β and PP2A Signaling. Yang C, Li X, Gao W, Wang Q, Zhang L, Li Y, Li L, Zhang L. Front Pharmacol 9 682 (2018)
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  24. A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice. Tamiru M, Takagi H, Abe A, Yokota T, Kanzaki H, Okamoto H, Saitoh H, Takahashi H, Fujisaki K, Oikawa K, Uemura A, Natsume S, Jikumaru Y, Matsuura H, Umemura K, Terry MJ, Terauchi R. New Phytol 210 1282-1297 (2016)
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