› 1rls

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF RNASE T1 COMPLEXED WITH THE PRODUCT NUCLEOTIDE 3'-GMP. STRUCTURAL EVIDENCE FOR DIRECT INTERACTION OF HISTIDINE 40 AND GLUTAMIC ACID 58 WITH THE 2'-HYDROXYL GROUP OF RIBOSE

Released:
DOI: 10.2210/pdb1rls/pdb
PDB entry 1rls coloured by chain, front view.
PDB entry 1rls coloured by chain, side view.
PDB entry 1rls coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Crystal structure of RNase T1 complexed with the product nucleotide 3'-GMP. Structural evidence for direct interaction of histidine 40 and glutamic acid 58 with the 2'-hydroxyl group of the ribose.
Gohda K, Oka K, Tomita K, Hakoshima T
J Biol Chem 269 17531-6 (1994)
PMID: 7912696

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 3GP 1 x 3GP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48.14Å b: 51.06Å c: 40.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided

Quick links

Citations

1 review citation

A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.
Steyaert J. (1997)
15 other articles

4 mentions without citation

Multiple structural alignment by secondary structures: algorithm and applications.
Dror et al. (2003)
3 more