› 1rxu

X-ray diffraction
3.1Å resolution

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-536992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Length: 253 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand PO4 18 x PO4
Ligand K 9 x K
Ligand THM 18 x THM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 156.244Å b: 97.655Å c: 161.453Å
α: 90° β: 118.23° γ: 90°
R-values:
R R work R free
0.213 0.212 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
1 more

1 mention without citation

Expression, purification, crystallization and preliminary X-ray structure analysis of Vibrio cholerae uridine phosphorylase in complex with thymidine.
Lashkov et al. (2012)