› 1rzm

X-ray diffraction
2.2Å resolution

Crystal structure of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) from Thermotoga maritima complexed with Cd2+, PEP and E4P

Released:
DOI: 10.2210/pdb1rzm/pdb
PDB entry 1rzm coloured by chain, front view.
PDB entry 1rzm coloured by chain, side view.
PDB entry 1rzm coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of the reaction complex of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Thermotoga maritima refines the catalytic mechanism and indicates a new mechanism of allosteric regulation.
Shumilin IA, Bauerle R, Wu J, Woodard RW, Kretsinger RH
J Mol Biol 341 455-66 (2004)
PMID: 15276836

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.43 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYH8 (Residues: 1-338; Coverage: 100%)
Gene names: TM_0343, aroF
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CD 3 x CD
Ligand PEP 2 x PEP
Ligand E4P 2 x E4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 71.581Å b: 144.215Å c: 141.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The enzymes of sialic acid biosynthesis.
Tanner ME. (2005)
3 more

8 mentions without citation

X-ray Scattering Studies of Protein Structural Dynamics.
Meisburger et al. (2017)
7 more