› 1s0i

X-ray diffraction
1.6Å resolution

Trypanosoma cruzi trans-sialidase in complex with sialyl-lactose (Michaelis complex)

Released:
DOI: 10.2210/pdb1s0i/pdb
PDB entry 1s0i coloured by chain, front view.
PDB entry 1s0i coloured by chain, side view.
PDB entry 1s0i coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
Amaya MF, Watts AG, Damager I, Wehenkel A, Nguyen T, Buschiazzo A, Paris G, Frasch AC, Withers SG, Alzari PM
Structure 12 775-84 (2004)
PMID: 15130470

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Trans-sialidase Chain: A
Molecule details ›
Chain: A
Length: 648 amino acids
Theoretical weight: 71.35 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q26966 (Residues: 2-635; Coverage: 99%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL, SIA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 54.296Å b: 129.443Å c: 54.316Å
α: 90° β: 107.94° γ: 90°
R-values:
R R work R free
0.165 0.164 0.187
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Advances in the biology and chemistry of sialic acids.
Chen et al. (2010)
18 more

17 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
16 more