› 1s16

X-ray diffraction
2.1Å resolution

Crystal Structure of E. coli Topoisomerase IV ParE 43kDa subunit complexed with ADPNP

Released:
DOI: 10.2210/pdb1s16/pdb
PDB entry 1s16 coloured by chain, front view.
PDB entry 1s16 coloured by chain, side view.
PDB entry 1s16 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase.
Bellon S, Parsons JD, Wei Y, Hayakawa K, Swenson LL, Charifson PS, Lippke JA, Aldape R, Gross CH
Antimicrob Agents Chemother 48 1856-64 (2004)
PMID: 15105144

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-539376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 4 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 43.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P20083 (Residues: 1-390; Coverage: 62%)
Gene names: JW2998, b3030, nfxD, parE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand SO4 2 x SO4
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 94.9Å b: 120.8Å c: 136.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.24
Expression system: Escherichia coli

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Citations

11 review citations

Challenges of antibacterial discovery.
Silver LL. (2011)
10 more

23 mentions without citation

Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.
Forterre et al. (2009)
22 more