Function and Biology Details
Reactions catalysed:
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
DNA (containing Sp-methylphosphotriester) + protein L-cysteine = DNA (without Sp-methylphosphotriester) + protein S-methyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding
- Methylated-DNA-[protein]-cysteine S-methyltransferase, active site
- Winged helix-like DNA-binding domain superfamily
- Methylated DNA-protein cysteine methyltransferase, DNA binding domain
- Methylguanine DNA methyltransferase, ribonuclease-like domain
- Methylated DNA-protein cysteine methyltransferase domain superfamily
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional transcriptional activator/DNA repair enzyme Ada
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 19.77 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 180 amino acids
Theoretical weight: 19.77 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
- Canonical:
P06134 (Residues: 175-354; Coverage: 51%)
Sequence domains:
- 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain
- 6-O-methylguanine DNA methyltransferase, DNA binding domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-6A, RIGAKU RU300
Spacegroup:
P21
Expression system: Escherichia coli
