› 1sif

X-ray diffraction
2.18Å resolution

Crystal structure of a multiple hydrophobic core mutant of ubiquitin

Released:
DOI: 10.2210/pdb1sif/pdb
PDB entry 1sif coloured by chain, front view.
PDB entry 1sif coloured by chain, side view.
PDB entry 1sif coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Exploring sequence/folding space: folding studies on multiple hydrophobic core mutants of ubiquitin.
Benítez-Cardoza CG, Stott K, Hirshberg M, Went HM, Woolfson DN, Jackson SE
Biochemistry 43 5195-203 (2004)
PMID: 15122885

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 606-684; Coverage: 12%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 46.806Å b: 49.022Å c: 75.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Ubiquitin: a small protein folding paradigm.
Jackson SE. (2006)
14 other articles

2 mentions without citation

Relating destabilizing regions to known functional sites in proteins.
Dessailly et al. (2007)
1 more