› 1sty

X-ray diffraction
1.67Å resolution

THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE

Released:
DOI: 10.2210/pdb1sty/pdb
PDB entry 1sty coloured by chain, front view.
PDB entry 1sty coloured by chain, side view.
PDB entry 1sty coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease.
Keefe LJ, Sondek J, Shortle D, Lattman EE
Proc Natl Acad Sci U S A 90 3275-9 (1993)
PMID: 8475069

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.9 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
UniProt:
  • Canonical: P00644 (Residues: 83-208, 209-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41
Unit cell:
a: 46.952Å b: 46.952Å c: 62.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Not provided

Quick links

Citations

4 review citations

Helical membrane protein folding, stability, and evolution.
Popot et al. (2000)
3 more

1 mention without citation

Evolutionary origin of a secondary structure: π-helices as cryptic but widespread insertional variations of α-helices that enhance protein functionality.
Cooley et al. (2010)