› 1t1m

Electron Microscopy
12Å resolution

Binding position of ribosome recycling factor (RRF) on the E. coli 70S ribosome

Released:
DOI: 10.2210/pdb1t1m/pdb
PDB entry 1t1m coloured by chain, front view.
PDB entry 1t1m coloured by chain, side view.
PDB entry 1t1m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications.
Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J
Proc Natl Acad Sci U S A 101 8900-5 (2004)
PMID: 15178758
Related structures: EMD-1077

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160851 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Ribosome-recycling factor Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 21.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P84154 (Residues: 1-185; Coverage: 100%)
Gene name: frr
Sequence domains: Ribosome recycling factor
dodecamer fragment of double helix from 23S rRNA Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.85 KDa
Source organism: Escherichia coli
42-mer fragment of double helix from 16S rRNA Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12Å
Relevant EMDB volumes: EMD-1077

Quick links

Citations

13 review citations

What recent ribosome structures have revealed about the mechanism of translation.
Schmeing et al. (2009)
12 more

5 mentions without citation

Research progress on solutions to the sneak path issue in memristor crossbar arrays.
Shi et al. (2020)
4 more