› 1t2a

X-ray diffraction
1.84Å resolution

Crystal structure of human GDP-D-mannose 4,6-dehydratase

Released:
DOI: 10.2210/pdb1t2a/pdb
PDB entry 1t2a coloured by chain, front view.
PDB entry 1t2a coloured by chain, side view.
PDB entry 1t2a coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Walker JR, Vedadi M, Sharma S, Houston S, Wasney G, Loppnau P, Sundstrom M, Arrowsmith C, Edwards A, Oppermann U

Function and Biology Details

Reaction catalysed: 
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-mannose 4,6 dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 375 amino acids
Theoretical weight: 42.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60547 (Residues: 23-372; Coverage: 94%)
Gene name: GMDS
Sequence domains: GDP-mannose 4,6 dehydratase
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
1 bound ligand:
Ligand GDP 4 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 88.595Å b: 122.064Å c: 139.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.212
Expression system: Escherichia coli

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