› 1t2s

Solution NMR

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

Released:
DOI: 10.2210/pdb1t2s/pdb
PDB entry 1t2s coloured by chain, ensemble of 10 models, front view.
PDB entry 1t2s coloured by chain, ensemble of 10 models, side view.
PDB entry 1t2s coloured by chain, ensemble of 10 models, top view.
Source organism: Drosophila melanogaster
Primary publication:
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.
Lingel A, Simon B, Izaurralde E, Sattler M
Nat Struct Mol Biol 11 576-7 (2004)
PMID: 15156196

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114626 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.93 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VUQ5 (Residues: 602-720; Coverage: 10%)
Gene names: AGO2, CG7439
Sequence domains: PAZ domain
Structure domains: paz domain
5'-D(*CP*TP*CP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.44 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: MOLECULAR DYNAMICS, SIMULATED ANNEALING
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

66 review citations

Biogenesis of small RNAs in animals.
Kim et al. (2009)
65 more