› 1t6d

X-ray diffraction
2.15Å resolution

MIRAS phasing of the Aquifex aeolicus Ppx/GppA phosphatase: crystal structure of the type II variant

Released:
DOI: 10.2210/pdb1t6d/pdb
PDB entry 1t6d coloured by chain, front view.
PDB entry 1t6d coloured by chain, side view.
PDB entry 1t6d coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural characterization of the stringent response related exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family.
Kristensen O, Laurberg M, Liljas A, Kastrup JS, Gajhede M
Biochemistry 43 8894-900 (2004)
PMID: 15248747

Function and Biology Details

Reactions catalysed: 
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Guanosine 5'-triphosphate,3'-diphosphate + H(2)O = guanosine 3',5'-bis(diphosphate) + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ppx/GppA phosphatase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.13 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67040 (Residues: 1-312; Coverage: 100%)
Gene names: aq_891, ppx
Sequence domains: Ppx/GppA phosphatase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand TRS 1 x TRS
2 modified residues:
Ligand MSE 8 x MSE
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 54.461Å b: 83.565Å c: 69.764Å
α: 90° β: 97.39° γ: 90°
R-values:
R R work R free
0.207 0.206 0.255
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mitochondrial retrograde signaling.
Liu et al. (2006)
4 more

1 mention without citation

Crystal structure of a soluble fragment of poliovirus 2CATPase.
Guan et al. (2018)