› 1tez

X-ray diffraction
1.8Å resolution

COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS

Released:
DOI: 10.2210/pdb1tez/pdb
PDB entry 1tez coloured by chain, front view.
PDB entry 1tez coloured by chain, side view.
PDB entry 1tez coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair.
Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO
Science 306 1789-93 (2004)
PMID: 15576622

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-506553 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Deoxyribodipyrimidine photo-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 53.47 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli
UniProt:
  • Canonical: P05327 (Residues: 2-475; Coverage: 98%)
Gene names: phr, phrA, syc1392_c
Sequence domains:
Structure domains:
5'-D(*AP*TP*CP*GP*GP*CP*T*(TCP)P*CP*GP*C)-3' Chains: I, K
Molecule details ›
Chains: I, K
Length: 11 nucleotides
Theoretical weight: 3.26 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(P*CP*GP*AP*AP*GP*CP*CP*GP*A)-3' Chains: J, L
Molecule details ›
Chains: J, L
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*CP*GP*C)-3' Chains: M, O
Molecule details ›
Chains: M, O
Length: 4 nucleotides
Theoretical weight: 1.17 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(P*GP*CP*CP*GP*A)-3' Chains: N, P
Molecule details ›
Chains: N, P
Length: 5 nucleotides
Theoretical weight: 1.51 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand MG 2 x MG
Ligand HDF 4 x HDF
1 modified residue:
Ligand TCP 2 x TCP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 173.815Å b: 88.505Å c: 161.622Å
α: 90° β: 90.11° γ: 90°
R-values:
R R work R free
0.205 0.205 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

The cryptochromes: blue light photoreceptors in plants and animals.
Chaves et al. (2011)
40 more

35 mentions without citation

DNA repair by reversal of DNA damage.
Yi et al. (2013)
34 more