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A large conformational change of the translocation ATPase SecA.

Osborne AR, Clemons WM, Rapoport TA
Proc Natl Acad Sci U S A 101 10937-42 (2004)
Cited: 108 times
EuropePMC logo PMID: 15256599

Abstract

The ATPase SecA mediates the posttranslational translocation of a wide range of polypeptide substrates through the SecY channel in the cytoplasmic membrane of bacteria. We have determined the crystal structure of a monomeric form of Bacillus subtilis SecA at a 2.2-A resolution. A comparison with the previously determined structures of SecA reveals a nucleotide-independent, large conformational change that opens a deep groove similar to that in other proteins that interact with diverse polypeptides. We propose that the open form of SecA represents an activated state.

Reviews - 1tf5 mentioned but not cited (6)

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  2. The Sec System: Protein Export in Escherichia coli. Crane JM, Randall LL. EcoSal Plus 7 (2017)
  3. Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon. Rusch SL, Kendall DA. Biochim Biophys Acta 1768 5-12 (2007)
  4. SecA: a potential antimicrobial target. Chaudhary AS, Chen W, Jin J, Tai PC, Wang B. Future Med Chem 7 989-1007 (2015)
  5. Use of synthetic signal sequences to explore the protein export machinery. Clérico EM, Maki JL, Gierasch LM. Biopolymers 90 307-319 (2008)
  6. Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity. Weaver DR, King GM. Int J Mol Sci 24 55 (2022)

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  17. Traffic jam at the bacterial sec translocase: targeting the SecA nanomotor by small-molecule inhibitors. Segers K, Anné J. Chem Biol 18 685-698 (2011)
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  21. Sec-dependent protein translocation across biological membranes: evolutionary conservation of an essential protein transport pathway (review). Stephenson K. Mol Membr Biol 22 17-28 (2005)
  22. The structural view of bacterial translocation-specific chaperone SecB: implications for function. Zhou J, Xu Z. Mol Microbiol 58 349-357 (2005)
  23. Protein Transport Across the Bacterial Plasma Membrane by the Sec Pathway. Smets D, Loos MS, Karamanou S, Economou A. Protein J 38 262-273 (2019)
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