› 1u6b

X-ray diffraction
3.1Å resolution

CRYSTAL STRUCTURE OF A SELF-SPLICING GROUP I INTRON WITH BOTH EXONS

Released:
DOI: 10.2210/pdb1u6b/pdb
PDB entry 1u6b coloured by chain, front view.
PDB entry 1u6b coloured by chain, side view.
PDB entry 1u6b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a self-splicing group I intron with both exons.
Adams PL, Stahley MR, Kosek AB, Wang J, Strobel SA
Nature 430 45-50 (2004)
PMID: 15175762

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140468 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
U1 small nuclear ribonucleoprotein A Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
197-MER Chain: B
Molecule details ›
Chain: B
Length: 197 nucleotides
Theoretical weight: 64.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(*AP*AP*GP*CP*CP*AP*CP*AP*CP*AP*AP*AP*CP*CP*AP*GP*AP*CP*GP *GP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(*CP*AP*(5MU))-3' Chain: D
Molecule details ›
Chain: D
Length: 3 nucleotides
Theoretical weight: 910 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand K 5 x K
Ligand MG 13 x MG
3 modified residues:
Ligand GTP 1 x GTP
Ligand A23 1 x A23
Ligand 5MU 1 x 5MU

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4122
Unit cell:
a: 108.541Å b: 108.541Å c: 249.158Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

64 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
63 more

35 mentions without citation

RNA structure: the long and the short of it.
Holbrook SR. (2005)
34 more