Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
MazEF toxin-antitoxin complex (preferred)
PDBe Complex ID:
PDB-CPX-142412 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoribonuclease toxin MazF
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 11.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
Length: 110 amino acids
Theoretical weight: 11.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0AE70 (Residues: 2-111; Coverage: 99%)
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
Antitoxin MazE
Molecule details ›
Chain: C
Length: 85 amino acids
Theoretical weight: 9.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Antidote-toxin recognition MazE, bacterial antitoxin
Structure domains: Pemi-like Protein 1; Chain: D
Length: 85 amino acids
Theoretical weight: 9.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0AE72 (Residues: 1-82; Coverage: 100%)
Sequence domains: Antidote-toxin recognition MazE, bacterial antitoxin
Structure domains: Pemi-like Protein 1; Chain: D
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X9A
Spacegroup:
C2221
Expression system: Escherichia coli BL21(DE3)
