PDB 1uhk experiment details and validation ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI

X-ray diffraction

Source organism: Aequorea victoria

Resolution: 1.6Å

Reported R values:

R R_free R_work

0.16 0.19 0.16

Entry percentile scores

X-ray diffraction

Sample information

Author description:

Crystal structure of n-aequorin

Source organism:

Aequorea victoria

Expression system:

Escherichia coli

Validation information

Metric	Description
Bond angles in protein, DNA, RNA molecules	14 outlier(s) of 4222 (%)
Bond lengths in protein, DNA, RNA molecules	0 outlier(s) of 3122 (%)
Electron density fit in protein, DNA, RNA molecules	13 outlier(s) of 382 (%)
Ramachandran outliers in protein molecules	1 outlier(s) of 378 (%)
Sidechain rotamer outliers in protein molecules	12 outlier(s) of 318 (%)

Experimental information

X-ray source:

SPRING-8 BEAMLINE BL44XU

Dataset statistics

Metric	Value	Source
Cell dimensions	a: 34.223 Å b: 57.038 Å c: 57.768 Å α: 72.35° β: 79.97° γ: 67.89°	Depositor
Number of reflections	47362	Xtriage(Phenix)
Test set size	4.99%	EDS
Data completeness	93.3%	EDS
EDS resolution	1.6Å - 33.3Å	EDS
<I/σ(I)>	3.1	Xtriage(Phenix)
Twinning statistics	<\|L\|> = 0.5 , <L²> = 0.34	Xtriage(Phenix)
Spacegroup	P 1	Depositor
Possible (pseudo-) translation	The largest off-origin peak in the Patterson function is 8.18% of the height of the origin peak. No significant pseudotranslation is detected.	Xtriage(Phenix)
Wilson B	14 Å²	Xtriage(Phenix)

Refinement statistics

Metric	Value	Source
Refinement software	REFMAC	DCC
Bulk solvent B	50 Å²	EDS
Bulk solvent k	0.39 e^-/Å³	EDS
Fo-Fc correlation	0.956	EDS
R value (DCC)	0.1756	DCC
R_free (DCC)	0.2089	DCC
R value (EDS)	0.177	EDS

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1uhk @ RCSB
1uhk @ PDBj
1uhk @ PDBSum
1uhk @ Proteopedia

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