› 1uoz

X-ray diffraction
1.1Å resolution

Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with thiocellopentaose at 1.1 angstrom

Released:
DOI: 10.2210/pdb1uoz/pdb
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Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mycobacterium tuberculosis strains possess functional cellulases.
Varrot A, Leydier S, Pell G, Macdonald JM, Stick RV, Henrissat B, Gilbert HJ, Davies GJ
J Biol Chem 280 20181-4 (2005)
PMID: 15824123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 33.08 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79G13 (Residues: 88-380; Coverage: 77%)
Gene names: Rv0062, cel6, celA, celA1
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: SSG, GLC
Ligand SSG 2 x SSG
Ligand GLC 1 x GLC
3 bound ligands:
Ligand SGC 1 x SGC
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 61.94Å b: 92.627Å c: 46.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.112 0.13
Expression system: Escherichia coli

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Citations

5 review citations

Genome analyses highlight the different biological roles of cellulases.
Mba Medie et al. (2012)
4 more

3 mentions without citation

Modeling of loops in proteins: a multi-method approach.
Jamroz et al. (2010)
2 more

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