1uw4

X-ray diffraction
1.95Å resolution

The structural basis of the interaction between nonsense mediated decay factors UPF2 and UPF3

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-189859 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of nonsense transcripts 3B Chains: A, C
Molecule details ›
Chains: A, C
Length: 91 amino acids
Theoretical weight: 10.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZI7 (Residues: 50-140; Coverage: 19%)
Gene names: RENT3B, UPF3B, UPF3X
Sequence domains: Smg-4/UPF3 family
Structure domains: Alpha-Beta Plaits
Regulator of nonsense transcripts 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 248 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HAU5 (Residues: 768-1015; Coverage: 20%)
Gene names: KIAA1408, RENT2, UPF2
Sequence domains: MIF4G domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 70.306Å b: 100.178Å c: 153.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.227
Expression system: Escherichia coli BL21(DE3)